Randolph Faustino, PhD, Lab

 

Primary Research Focus

Dr. Faustino Bio

The Faustino lab investigates systems biology of the pluripotent nucleus, and how specific nuclear proteins orchestrate the intricate signaling framework underlying stem cell identity and function. Our approach integrates next generation sequencing and bioinformatic analysis with cell culture, molecular biology, and biochemistry techniques to resolve the molecular interactome that gives rise to the unique cellular properties associated with pluripotency. Ultimately, elucidating systems biology functions of the nucleus in a stem cell context is critical to our understanding of pluripotent biology for precise reparative and therapeutic strategies.

 

Peter Vitiello
  • Assistant Scientist, Children’s Health Research Center
  • Assistant Professor, Department of Pediatrics, Sanford School of Medicine at the University of South Dakota
  • Bachelor of Science in Microbiology at the University of Manitoba, Manitoba, Canada
  • Doctor of Philosophy in Physiology at the University of Manitoba, Manitoba, Canada
  • Postdoctoral Fellow at Mayo Clinic, Rochester, MN
   


Behind the research

Nucleoporins (nups) are a group of highly conserved eukaryotic proteins that comprise the nuclear pore complex. This intricate, megadalton structure resides within the nuclear envelope and links the cytoplasmic and nuclear compartments, acting as the main gateway essential to nucleocytoplasmic transport. Diverse and alternative functions for individual nups apart from their role in molecular trafficking have been identified that range from epigenomic control to directing nuclear mobility, all with developmental impacts and implications for disease etiology.

Stem cells provide a unique opportunity to study nups in a naïve functional background. In addition to being the most prominent feature of a stem cell, the nucleus is highly plastic prior to differentiation and its malleable transcriptome is part of a unique interplay of internal and external nuclear mechanisms that dictate downstream functionality of the stem cell and its ability to acquire distinct and specific fates. A systems biology-based inspection of the expanding functional repertoire of nups in a pluripotent setting provides fundamental insights into underlying molecular network relationships that drive stem cell biology. This understanding is critical for knowledge breakthroughs, with the ultimate goal of advancing strategies for precision-based medicine to treat developmental disease.

 

Resources Positions Available
Access Dr. Faustino's publications here. If you have an intense scientific curiosity, an interest in advancing the areas of stem cell and nuclear systems biology, and can see yourself as part of a dynamic and exciting team of scientists at Sanford Research, please contact Dr. Faustino for more information.
Meet members of the Faustino lab here.  
 

Contact Us:  
Phone: (605) 312-6443
Fax: (605) 312-6071
Email: randolph.faustino@sanfordhealth.org

 

Lab Projects

  • Epigenomic control of the molecular program underlying cardiac development
  • Regulatory mechanisms of three dimensional genome dynamics
  • Nuclear mobility and its role in development

 

Lab News 

November 2016, Dr. Randy Faustino joined Children’s Health at Sanford Research and opened his lab.