Roux Lab

Roux

Primary research focus

Kyle J. Roux, PhD

Our laboratory investigates the structure and function of the mammalian nuclear envelope in health and disease. We have identified and are functionally characterizing several novel members of the LINC-complex. These proteins are retained on the outer nuclear membrane where they appear to perform specific roles in specialized cell types. We are also investigating the mechanisms by which defects in a single constituent of the nuclear lamina, the intermediate filament scaffold inside the nucleus, lead to a wide variety of diseases called laminopathies. These studies are supported by the BioID method to screen for interacting and proximate proteins.
  • Executive Director of Biomedical Sciences, Sanford Research
  • Scientist and Co-Director, Children's Health Research Center
  • Associate Professor, Department of Pediatrics, Sanford School of Medicine, University of South Dakota

Behind the research

Defects in a cellular structure termed the nuclear envelope are associated with a myriad of diverse diseases, collectively called nuclear envelopathies. Most of these disorders clinically manifest during the first two decades of life and include muscular dystrophy, cardiomyopathy, lipodystrophy, dystonia, neuropathy, skeletal defects, and progeria. The nuclear envelope is situated at a critical juncture in the cell, both intimately associated with the genome and responsible for connecting it to the rest of the cell. While it is clear that mutations in genes encoding protein constituents of the nuclear envelope underlie these diseases, the exact mechanisms remain largely unknown. In part, these nuclear envelopathies involve a nuclear envelope structure called the LINC-complex that is responsible for linking the nucleoskeleton to the cytoskeleton.

Resources
Access Dr. Roux's publications here.
Contact Information
Telephone: (605) 312-6418
Fax: (605) 328-0401
Email: Kyle.Roux@sanfordhealth.org

 

Current projects

Development and application of BioID

Characterization of novel LINC-complex constituents

Investigating the mechanisms of disease for the laminopathies

Lab News

April 2016, A research publication from the Roux Lab was recently recommended for Faculty of 1000 as being of special significance in its field. F1000Prime publishes recommendations of articles in biology and medicine from a faculty of around 10,000 scientists and clinical researchers aimed at highlighting highly significant research. The article entitled “An improved smaller biotin ligase for BioID proximity labeling” was recently published in the journal Molecular Biology of the Cell.

March 2016, The lab of Dr. Kyle Roux recently published a study entitled ” An improved smaller biotin ligase for BioID proximity labeling” in the journal Molecular Biology of the Cell. This study introduces improvements to BioID, a method invited by Dr. Roux that uses a promiscuous biotin ligase to detect protein-protein interactions in living cells.

January 2016, Dr. Kyle Roux and members of his lab recently co-authored a book chapter in Methods in Enzymology entitled “BioID Identification of Lamin-Associated Proteins.” This chapter discusses the use of BioID, a research technique developed by Dr. Roux, to elucidate novel lamin-interacting proteins and its applications in a broad range of biological systems, and provides detailed protocols to guide this new and innovative applications.