Kyle Roux, PhD, Lab

Roux

Primary Research focus

Dr. Roux Bio

Our laboratory investigates the structure and function of the mammalian nuclear envelope in health and disease. We have identified and are functionally characterizing several novel members of the LINC-complex. These proteins are retained on the outer nuclear membrane where they appear to perform specific roles in specialized cell types. We are also investigating the mechanisms by which defects in a single constituent of the nuclear lamina, the intermediate filament scaffold inside the nucleus, lead to a wide variety of diseases called laminopathies. These studies are supported by the BioID method to screen for interacting and proximate proteins.
  • Senior Director of Biomedical Sciences, Sanford Research
  • Scientist and Co-Director, Children's Health Research Center
  • Associate Professor, Department of Pediatrics, Sanford School of Medicine, University of South Dakota

Behind the research

Defects in a cellular structure termed the nuclear envelope are associated with a myriad of diverse diseases, collectively called nuclear envelopathies. Most of these disorders clinically manifest during the first two decades of life and include muscular dystrophy, cardiomyopathy, lipodystrophy, dystonia, neuropathy, skeletal defects, and progeria. The nuclear envelope is situated at a critical juncture in the cell, both intimately associated with the genome and responsible for connecting it to the rest of the cell. While it is clear that mutations in genes encoding protein constituents of the nuclear envelope underlie these diseases, the exact mechanisms remain largely unknown. In part, these nuclear envelopathies involve a nuclear envelope structure called the LINC-complex that is responsible for linking the nucleoskeleton to the cytoskeleton.

Resources
Access Dr. Roux's publications here.
Contact Information
Telephone: (605) 312-6418
Fax: (605) 328-0401
Email: Kyle.Roux@sanfordhealth.org

 

Current projects

Development and application of BioID

Characterization of novel LINC-complex constituents

Investigating the mechanisms of disease for the laminopathies

Lab News

April 2017 Kyle Roux, PhD, was awarded a research grant from BioSNTR for his project, "In Planta BioID to detect protein-protein interactions in plant cells."

April 2017 A team of Sanford physicians and scientists led by Drs. Megan Landsverk, Kyle Roux, and Jill Weimer published a research article in American Journal of Medical Genetics that investigated the molecular consequences of PHOX2B mutations in patients with congenital central hypoventilation syndrome. "Nonsense pathogenic variants in exon 1 of PHOX2B lead to translational reinitiation in congenital central hypoventilation syndrome."

December 2016 Dr. Kyle Roux and Birenda KC published "The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity" in Scientific Reports. The study describes how mechanical connections that connect the nucleus to the cell impact the cell's ability to regulate gene expression in response to mechanical stimuli from the cell's exterior. https://www.ncbi.nlm.nih.gov/pubmed/?term=The+mammalian+LINC+complex+regulates+genome+transcriptional+responses+to+substrate+rigidity

Decemeber 2016 Dr. Kyle Roux and Birenda KC, USD Graduate Student, attended the annual meeting of the American Society for Cell Biology in San Francisco, CA. They presented their work on a novel lamin-associated kinase in a session titled Nuclear Lamins and Laminopathies.

October 2016, Dr. Roux received the Blue Flame Award from Addgene, a nonprofit plasmid repository, for receiving over 3,700 requests his BioID plasmids from researchers around the world.

News Archive