Application of genetic and molecular biological approaches is essential for understanding developmental processes and pediatric disorders. These techniques permit the identification and characterization of genes, analysis of gene expression, determination of the functions and interactions of these genes, and expression of reporter genes and proteins to allow cellular localization and physiological characterization. The mission of the Molecular Biology Core is to provide infrastructure, expertise and support in molecular biology applications for COBRE project leaders and other Sanford Research researchers.
Email: Molecular Biology Core
Director Molecular Biology Core
The main goal of Molecular Biology Core is to provide quality services and training in molecular biology and genetics assays that are conducted with state-of-the-art technologies. The Molecular Biology Core facility will provide assistance with experimental design, resource assessment, sample preparation, assay performance, and data management and analysis. Core personnel will provide detailed and customized protocols of the methods that will be used for manuscripts or funding applications. By providing these personalized services in-house, the core director will facilitate the execution of each COBRE project. The state-of-the-art, high-throughput equipment supports a wide range of genetic analysis services, including RNA extraction and quality analysis, quantitative PCR (Q-PCR) analysis as well as initial data analysis. Q-PCR service is predominantly based on hybridization probe (e.g. TaqMan) analysis, but SYBR green approaches are supported. Starting with RNA, the facility will utilize primers from investigators, or design custom primers and perform Q-PCR. The facility provides control primers and assists with experimental design, as well as providing basic relative quantitation analysis. These in-house services provided by the Molecular Biology Core are complementary to the services offered through the Functional Genomics Core and Microarray Core at the Sanford-Burnham Medical Research Institute (SBMRI, La Jolla, CA). The core will serve as an interface between the investigator and these cores at SBMRI.