Development and application of BioID

We developed BioID to overcome methodological challenges in our studies on the structure and function of the nuclear envelope in health and disease. First applied to a disease-associated nuclear lamin, we demonstrated that BioID is effective at identifying proximate and interacting proteins. Ongoing studies include the application of BioID to uncover the mechanisms of disease for the nuclear envelopathies, expansion of its application to a myriad of subcellular domains, and further optimization of the BioID system.