The mission of the Flow Cytometry (FCM) core is to assist all the investigators in Sanford community and their regional collaborators in performing cutting-edge flow cytometry and cell sorting. The FCM core serves both of the basic science and the translational research. The core owned by Sanford Research is partly supported by a NIH COBRE grant for Cancer Biology Research Center at Sanford Research.
||Alexei Savinov, MD, PhD
Director of Flow Cytometry Core
Maudi Killian, BS
Senior Laboratory Technician, Core Technical Contact
- Provides instrumentation: See next section for the list of the instruments and specifications. The FCM core has developed user registration protocols and standard operation procedures for each instrument. The process will be initiated by contacting the FCM core by an e-mail.
- Cellular sorting service by the flow cytometry (semi self-operated sorting): FCM core provides the set-up for the user's particular cell sorting experiment in every session. We expect users to conduct sample application and shut-down (according to the SOP we will provide). The core will provide the trainings up to 3-times for the sample application part.
- Provides flow cytometry consultation: Various aspects of flow cytometry including designing experiment protocols, sample preparation methods, assessing the project feasibility, interpretation of the flow cytometry data and preparation of data presentation.
- Recommended 20-min introduction for flow cytometry beginners: BD “Introduction to Flow Cytometry”
- Applications include cell cycle analysis (1D or 2D), assessment of apoptosis with various probes, cell number and viability assessment, dye dilution proliferation assays (such as CFSE dilution), multiparameter immunophenotyping (staining of cell surface and intracellular proteins), assessment of phosphorylation status of signaling proteins, cell functional studies using fluorescent probes (such as Calcium flux), multiplex bead assay and measuring proximity by fluorescence resonance energy transfer (FRET)
- BD Accuri C6 - (two-laser, 4-color) – TWO units, 24/7 for self-service to the internal users (in Sanford Center) and Monday - Friday, 10:00 - 20:00 for self-service to the external users (one training session required).
- Guava EasyCyte 8HT - (two-laser, 6-color), 24/7 for self-service to the internal users (in Sanford Center) and Monday - Friday, 10:00 - 20:00 for self-service to the external users (one training session required).
- BD FACS LSRFortessa SORP - (5-laser, 21-color, high-performance model with HTS), 24/7 for self-service to the qualified internal users and Monday - Friday, 10:00 - 20:00 for self-service to the qualified external users. After user registration, 2-4 training sessions are provided by FCM core. The trainings will be customized to user’s application. FCM core qualify the user for self operations at some point.
- BD FACSJazz - (three-laser, 6-color, installed in a biosafety cabinet), 4 sessions per week (Monday, Tuesday, Thursday, and Friday) are available for user’s semi self-operated sorting. Need to coordinate with FCM core for booking of each session at least 10 days in advance. Start-up (~ 1hr) will be conducted by core staff. The setting will be adjusted to the users’ application. Users will conduct sample application and shut-down (according to the SOP we provide).
- MACS magnetic bead separator - (manual) - 24/7, Applications includes the pre-enrichment of desired cell populations for flow cytometry sorting
- MACS tissue dissociator - 24/7
- EVOS FL Inverted fluorescence microscope - for sorter users to observe the pre- and post-sorted cells
- Cell culture facilities (Sanford Center room 1261) - for sorter users - 24/7, (CO2 incubator, low-speed centrifuge with sealed buckets, an inverted microscope, work BSC, refrigerator/freezer, sink)
- Workstations and software -24/7, Two workstations, installed with FlowJo, FCSExpress, CFlow, MS Office, Guava Soft and Modfit LT, are accessible with or without network connection